General description

Recombinant, human caspase-1 fused at the N-terminus to a His Tag™ sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Thornberry, N.A. 1994. Methods Enzymol. 244, 615.Thornberry, N.A., et al. 1992. Nature 356, 768.

Packaging

3000 u in Plastic ampoule

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (catNO=400025">Cat. No. 400025) per min at 30°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)